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Peer review Peer review information Nature Communications thanks Jos Pedro Friedmann Angeli and the other, anonymous, reviewer(s) for their contribution to the peer review of this work

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The culture medium was replaced at each time-point and the cells were then incubated with 110 l complete medium containing 10 l Cell Counting Kit (CCK)-8 reagent (Beijing Solarbio Science & Technology Co., Ltd.) for 90 min at 37C

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Additionally, in oxidative stress cells, Tripartite Motif Containing 22 (TRIM22) has been found to interact with Keap1 and accelerate the ubiquitination and degradation of NRF2, thereby inhibiting oxidative stress progression and activating ROS/AMPK/mTOR/autophagy signaling pathways