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Each pixel was transformed into a phasor plot according to the following equations: in which g i , j ( ) and s i , j ( ) are the x and y coordinates of the phasor plot, n and are the harmonic frequency and the angular frequency of excitation, respectively, and T is the repeat frequency of the acquisition

Its also the primary component in the flavor enhancer monosodium glutamate, so its found in certain processed foods as well

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( d ) NAO staining of mitochondrial mass in HT29 cells treated 5FU for 72 h, n = 3 biological replicates, with mean s.e.m

After 2 days, the medium was removed and replaced with prewarmed fresh RPMI-1640 with or without 10 mg/mL albumin