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Cells were viewed under a fluorescence microscope

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the total number of actin enriched spines (red) adjacent to VGLUT1 or Synapsin were counted and converted to a percentage as the percent correlation of treatment-induced spines to presynaptic markers is a strong indicator of ability to transmit excitatory signals

We also searched ClinicalTrials.gov, EudraCT, and conference abstracts for additional eligible studies and trial information

Definition and diagnostic criteria of clinical obesity