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Interestingly, S460A and S461A displayed about twofold enhancement in activity

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Circulation 1998

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Mechanisms of Drug Entry into Human Milk The amount of drug excreted into milk depends on a number of kinetic factors: 1) the lipid solubility of the drug, 2) the molecular size of the drug, 3) the blood level attained in the maternal circulation, 4) protein binding in the maternal circulation, 5) oral bioavailability in the infant, and the mother, and 6) the half-life in the maternal and infant's plasma compartments