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Protoplast Isolation and Transfection Two-week-old seedlings grown on 1/2 MS were used to isolate protoplasts as described previously with minor modifications ( Transcriptome Analysis Total RNA was extracted from 3-week-old plants with TRIzol (Invitrogen) and purified using a GeneJET plant RNA purification kit (Thermo Fisher Scientific)

Neurosci & Biobehav Rev

E., Zhang, C., Pollack, A

The primary aim of this study is to elucidate the molecular mechanisms underlying CP-induced ovarian injury, with a focus on oxidative stress, inflammatory signaling, and apoptotic pathways, focusing on nuclear factor-kappa B (NF-B), nuclear factor erythroid-2-related factor 2/heme oxygenase-1 (Nrf2/HO-1), Toll-like receptor 4 (TLR4), nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3 (NLRP3) inflammasome, silent information regulator-1 (SIRT1), and other signaling pathways involved in the pathogenesis of ovarian injury caused by CP

Brussels, BE

European Journal of Endocrinology, 139(5), 552561