Resorufin formation was monitored in 96-well plates by measuring absorbance at 562 nm (FLUOstar, BMG) or fluorescence (ex 571 nm/em 585 nm) (CLARIOstar, BMG) at room temperature
This property makes it appealing to those aiming to improve body composition and support weight loss efforts
4 Conclusion and prospect Microplastics can enter the organism through the respiratory system (e.g., inhalation, intratracheal instillation, and intranasal administration) and the digestive system (e.g., drinking water and oral ingestion), subsequently accumulating in multiple organs and inducing toxic effects in the liver, nervous system, lungs, kidneys, intestines, heart, and reproductive system
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[13] In the first step of the reaction, acyl-CoA synthetase catalyzes the transfer of adenosine monophosphate group (AMP) from an ATP molecule onto the fatty acid generating a fatty acyladenylate intermediate and a pyrophosphate group (PP i )
Materials and methods Materials All reagents were purchased from Merck Life Science Ltd., Hungary, unless otherwise indicated