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Knockout of NRF2 gene product was validated by immunoblotting

A decrease in glucose production indicates effective amylase inhibition, which can slow carbohydrate digestion and reduce blood sugar levels

due to continuous improvements, product packaging may vary slightly

the membranes were then washed several times with water and TBS (PBS, 0.05% Tween-20), a 1:5000 dilution of horseradish-peroxidase conjugated goat anti-rabbit antiserum was added, and the membranes further incubated for 1 h at room temperature

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