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Fluorescence spectroscopy studies of the binding of PREG and the related sulfated neurosteroid 3-hydroxy-5-pregnan-20-one sulfate, which differentially modulate NMDA and AMPA receptors, suggest that the differential effects of these sulfated neurosteroids on current flow may be related to their binding at the SIS2 and amino terminal domains of these receptors (178)

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Perseus software 62 (version 1.6.1.3) was used for calculation of the significance B (psigB) for each protein ratio with respect to the distance of the median of the distribution of all protein ratios as well as protein intensities 59