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The absorbance of the supernatant solution was measured spectrophotometrically at 410 nm

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High levels of IL-17 receptors and IL-17 positive T-cells have been identified in the synovial fluid of these patients

Finally, the tissue sections were washed thoroughly with PBS, counterstained with DAPI and imaged under a laser scanning confocal microscope (FV-1000, Olympus, Japan)

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