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The imidazole nitrogen of the histidine side chain donates one of the three nitrogen ligands to the copper, and the position of histidine at the second residue is what enables the deprotonated amide nitrogen of the preceding Gly-His peptide bond to also coordinate the copper

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[71] [75] It is not easy to eat uncooked liver, and extracts were developed as a concentrate of liver juice for intramuscular injection