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The PCR product was purified using a MinElute kit (Qiagen)

This sleep dysfunction likely both results from and contributes to other symptoms, creating a vicious cycle where poor sleep worsens pain sensitivity, which further disrupts sleep

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Activation of downstream effectors, including the mechanistic target of rapamycin complex 1 (mTORC1) and ribosomal protein S6 (S6), regulates protein synthesis, cell growth, and metabolism

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[DOI] [PMC free article] [PubMed] [Google Scholar] Grek CL, Zhang J, Manevich Y, Townsend DM, Tew KD